Tussilago farfara is a perennial plant native to Europe, used in herbal medicine. The substitute habitats, where it is a pioneer species, are exposed to heavy metal pollution. The aim of the study was to investigate the level of contamination with selected metals (Mn, Fe, Ni, Cu, Zn, Cd, Pb) at different sites and parts of the plant. Samples were collected from 33 sites located in southern and south-eastern Poland, in habitats with varying degrees of anthropopression, to assess whether the collected material may pose a threat to human health. Metal samples were determined by atomic absorption spectroscopy (AAS), and the bioconcentration factor (BCF) was also calculated. The study showed clear organ-specific differences in metal distribution: mean Mn and Fe concentrations were higher in underground parts, whereas Ni, Zn, Cd and Pb were slightly higher in inflorescences; Cu did not differ markedly between plant parts. Cadmium was the only element showing intensive accumulation (BCF = 1.44), whereas the remaining elements showed weak to medium accumulation. For most of the analytes, plant’s parts were characterised by an average level of accumulation (BCF: Cu 0.76, Ni 0.13, Zn 0.65, Pb 0.47, Mn 0.4, Fe 0.13). An intensive degree of accumulation was detected only for cadmium (BCF 1.44). Cadmium was the only element for which concentrations in plant tissues were, on average, higher than in soil and this was consistent with BCF values above 1. In contrast, copper did not show statistically significant differences between soil and plant tissues. In some plant samples, the concentration of Pb and Cd exceeded the permissible values given for herbs in the Commission Regulation of 2023. Our results indicate that collecting coltsfoot from natural habitats is not recommended due to the risk of accumulation of heavy metals, especially cadmium.