The study aimed to determine the effect of antibiotic administration and vaccination on the level of selected mineral elements in the yolk sac and blood serum of turkeys. Turkey poults were divided into 8 experimental groups: a control group (C) receiving no antibiotics or coccidiostats; groups receiving either enrofloxacin (E) or doxycycline (D) for the first 5 days post-hatch; and a group administered monensin (M) for 56 days of life. The levels of Ca, P, Mg, Cu, Zn, and Fe were determined in yolk sac and blood serum samples. The results showed that poults utilise minerals from the yolk sac during the first days post-hatch, as indicated by their decreasing levels. Enrofloxacin treatment did not affect yolk sac levels of P and Zn, but it significantly impaired their absorption into circulation, with effects persisting for 7 weeks post-treatment. Both enrofloxacin and doxycycline administration transiently reduced Cu and Fe levels, although compensatory increases in serum concentrations were observed following treatment cessation. Long-term monensin exposure led to clinically relevant reductions in serum calcium levels.