The aim of the research was to determine the character of interactions between different groups of bees and the influence on defense and hygiene behavior of a bee colony as a whole. Two groups of bees were compared: aggressive and docile. Observations were conducted on synthetic experimental nuclei colonies. They were created according to a model which uses five nuclei colonies: 2 homogenous ones, with one consisting of 100% aggressive bees and the second one consisting of 100% docile bees, and three mixed ones in which aggressive and docile bees were combined in different proportions (20, 50, 80%). Stinging tests and hygienic testes (removing the dead pupae and lined paper) were carried out on experimental nuclei colonies. The tests indicated that aggressive bees had a bigger influence on defense behavior as they showed behavioral dominance depending on the breed of mixed bees. On the other hand, bee mixing had no conclusive influence on hygienic behavior depending on the breed of mixed bees. In homogenous nuclei colonies the bees which removed the lined paper faster were also faster in removing dead pupae, and those which removed the lined paper more slowly, were slower in removing dead pupae. In mixed nuclei colonies the pace of removing the lined paper was not connected with the proportion of bees in a nuclei colony but it depended on their interaction.