The objective of this study was to determine fish species differences in fatty acid profile of the fat of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish so as to compare the consumer health benefits they provide. Fatty acid composition range was as follows: saturated fatty acids (SFA), 16.32%–32.96%; monounsaturated fatty acids (MUFA), 41.84%–55.31%; and polyunsaturated fatty acids (PUFA), 13.4%–26.31%. The total content of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in 100 g of muscle tissue of the fish examined in the current study was 62.61 mg for grass carp, 150.51 mg for wels catfish, 488.67 mg for bighead carp, and 619.06 mg for Siberian sturgeon. The ratios of n-6/n-3 (0.44–1.72) and PUFA/SFA (0.45–1.61) in the fat of the fish analyzed were beneficial.