In this study, an investigation on the effect of the magnetic field on interphase phenomena influencing chromatographic separation was performed using thin-layer chromatography method. The presence of perpendicular to plane of the plate and the direction of chromatogram development magnetic field influences the interaction among all components of the chromatographic system. In consequence, the retention and efficiency of the investigated compounds in the given system should be altered. In this article, the effect of the field presence regarding retention mechanism, distance of development, and particle size of stationary phase for chosen derivatives of 2-(2,4-dihydroxyphenyl))-1,3,4-thiadiazoles is being investigated. The alteration of each abovementioned parameter affects the retention change caused by the field. It was also observed that the retention changes depend also on the structure of the studied compound, which may be a useful information for investigations on properties of newly synthesized compounds.