he aim of the study was to evaluate the effects of using organic mulches: barley straw, chipped pine bark, foresthumus, compost, cow manure, commercial peat moss, and commercial mycorrhizal substrate on the growth andyield ofŠampion apple trees produced on M26, P2, M9, and P22 rootstocks. The investigations were conductedusingŠampion apple trees grown on chosen rootstocks and substrates: barley straw, chipped pine bark, foresthumus, compost, cow manure, commercial peat moss substrate, and commercial mycorrhizal substrate. Appletrees grown on all analysed rootstocks reached the greatest height on compost and cow manure mulches, and thebiggest trunk diameter was achieved by trees growing on the straw mulch. The greatest number (P < 0.05) offruits was produced by trees growing on the forest humus mulch, especially those grafted on rootstocks M9 andP22. The forest humus, compost, and cow manure mulches had a beneficial effect on the content of mineralelements in the soil and leaves ofŠampion trees growing on all rootstocks. The humus, compost, and cowmanure mulches had a positive effect on the growth of the apple trees. The organic mulches increased thenumber apples and the content of minerals in the soil and leaves