The aim of this study was to determine the effect of addition of alfalfa protein concentrate (APC) at the dose of 15 g or 30 g per 1 kg on the chemical composition, fatty acid profile, dietary value, and antioxidant status in muscles and serum of Ross 308 chickens. The experiment involved 150 1-day-old Ross 308 broiler chickens allocated into 3 groups in 5 replications (10 birds per pen). A 1-way system with 2 levels of APC, 15 g or 30 g per 1 kg of a complete diet, was used. Group C receiving a standard feed mixture without APC was the control. The addition of 15 and 30 g APC increased the CP content in the breast muscle and in the thigh muscle, and reduction in the cholesterol and fat level was noted. Higher content of polyunsaturated fatty acids from the n-6 group was noted in the breast muscles of the 30-g APC–supplemented chickens. The thigh muscles with APC were characterized by more favorable values of the atherogenic index, thrombotic index, and hypocholesterol-to-hypercholesterol ratio.The supplementation with 30 g APC reduced the cholesterol and triacylglycerol levels, increased the high-density lipoprotein level, and decreased the low-density lipoprotein level and improved the antioxidant parameters in plasma (increase in superoxide dismutase and in glutathione peroxidase and reduction of malondialdehyde), compared with group C. The results of this study indicate that the supplementation with 30 g APC improved the metabolic functions of the organism, meat resistance to oxidative processes, and the composition and profile of fatty acids. Therefore, APC can be a potential alternative to synthetic feed additives and soya protein in production of healthier poultry meat.