Durum wheat is the tenth most valuable crop on a global scale. The aim of this study was to compare the phenotypic variation of T. durum accessions of different origin with contemporary spring cultivars of this cereal species. One hundred and two accessions and 12 contemporary cultivars of Triticum durum Desf. as well as Kamut® wheat (T. turanicum), a Triticum species closely related to T. durum, were analyzed. The aim of this study was to describe the degree of variation in the phenotypic traits of grain and selected traits associated with technological quality. The examined genotypes were characterized by considerable phenotypic variation, and they can be a valuable source of material for genetic recombination in durum wheat breeding. The analyzed accessions were characterized by a higher average content of protein (16.48 vs. 14.56%) and wet gluten (38.04 vs. 32.07%), higher Zeleny sedimentation values (69.7 vs. 60.4ml), and higher flour strength (W index values of 404.64 vs. 353.47) than the reference cultivars. The kernels of the evaluated accessions and cultivars did not differ significantly in average crease depth, but significant differences were observed in the values of descriptors directly linked with kernel size, especially kernel image area and minimal Feret diameter. The traits responsible for the processing suitability of grain were more strongly correlated with color descriptors than shape descriptors, which suggests that color parameters can be used to select high-quality breeding material. The analyzed accessions have two major weaknesses, namely relatively low yields (22.6 dt ha-1 on average) and undesirable grain color, indicative of low carotenoid concentration. The accessions deposited in gene banks do not meet the relevant agronomic requirements. However, both grain yield and carotenoid concentration are polygenic traits which can be improved if desirable combinations of QTLs are assembled in breeding lines and cultivars.