In the present study freeze- and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers (inulin, oligofructose, Nutriose®) and maltodextrin were obtained and analyzed for physicochemical and biological properties. Fermentation and carrier type affected the powders' quality to a greater extent than drying techniques. Higher betalains content was noted for non-fermented juice powders, while ferulic and syringic acids' derivatives for their fermented-juice-based counterparts. Oligofructose induced hydroxymethyl-L-furfural formation, but also together with inulin resulted in products with the strongest antioxidant capacity. Nutriose® had the greatest in vitro antiproliferative activity towards human leukemia cell lines, unlike oligofructose which was shown to stimulate their growth. Overall, fermentation that led to beetroot matrix modification and carrier type affected powders’ quality toward their improved potential functional properties.