Recently, the ecological awareness of society and the need to take care of the natural environment have increased significantly. There is also an urgent problem of searching for new, environmentally friendly, and safe for people plant protection techniques using biological preparations, which reduce the intensive and cause significant health problems chemical protection. The study was conducted in a foil tunnel on the ‘Red House’ cultivar roses grown using an adapted method with shoot bending. Maintaining their health under tunnel conditions is often problematic. The study determined the effect of biopreparations on plant health (disease index), photosynthesis parameters, and gas exchange, as well as the species composition of fungi inhabiting roses. The preparations used did not negatively affect the process of photosynthesis and gas exchange. Among the 25 species of fungi obtained from its organs, the polyphagous species Botrytis cinerea dominated; the organs were very often colonized by fungi from the genera Fusarium, Phoma, and Alternaria alternata. The highest concentration of the Biosept 33 SL biopreparation shows a protective effect similar to that of the preparations used in chemical protection, and the degree of leaf blade infection shows a similar level.