The aim of this study was to evaluate the feasibility of using cross-linked aggregates of versatile peroxidase (CLEAs-VP/Ba) derived from Bjerkandera adusta strain CCBAS 930 for bisphenol A (BPA) biotransformation. To optimize VP/Ba biosynthesis in stationary liquid cultures (28 °C), BPA (40 mg/mL) was added, and oxidation efficiency of 10 mM 2,6-dimethoxyphenol (2,6-DMP) was measured at various intervals (days 3, 7, 10, 14, 18, 20, and 26). The tests were conducted in 0.1 M acetate buffer at pH 3.0 and 5.0, with and without Mn2+. Maximum activity for VP/Ba was determined on day 14, after which the culture fluid was separated from the mycelium and ammonium sulfate (80 %) and 72 mM glutaraldehyde was added to prepare CLEAs-VP/Ba (22 h, 150 rpm, 30 °C). The activity recovery (ARCLEAs-VP/Ba) and aggregation yield (AYCLEAs-VP/Ba) were 11.65 % and 48.95 %, respectively. The BPA (10 mg/L) removal efficiency of CLEAs-VP/Ba and 10 mM H2O2 was measured spectrophotometrically after 30, 60, 90 and 120 min. Results showed that CLEAs significantly increased the activity of VP/Ba peroxidase, achieving an 83 % BPA removal efficiency after 120 min (4.15 mg/h). Moreover, CLEAs-VP/Ba-treated samples exhibited reduced biotoxicity, with no observed phytotoxicity. The treatment also mitigated long-term adverse effects, such as genotoxicity. In case of estrogenic activity, no significant decrease was observed after the use of CLEAs-VP/Ba.