Modern methods of cultivating trees in a nursery plants require nursery stock of very good quality, free from viruses, with an extensive root system, strong growth and, most importantly, with the correct structure of the tree crown, i.e. the number of lateral shoots. High-quality planting material, properly branched, determines the productive efficiency of the trees in a nursery in subsequent years of cultivation and its profitability. The research was conducted in 2017–2019 at a private nursery farm near Lublin. The purpose of the research was to analyze the effect of mixtures of biostimulants with growth regulators of natural origin on the effectiveness of chemical treatment to stimulate the emer-gence of lateral shoots in apple trees of the Gloster. The study showed that the height of maiden apple trees of the Gloster. significantly depended on the concentration of growth regulators. The best results were achieved using a mixture of AGRIMIX PRO (35 ml) and Maxifruit, producing trees 6% taller thanthe control. The number of side shoots, the average length of one shoot and the sum of the lengths of side shoots significantly depended on the number of growth regulator applications. Double applica-tion of AGRIMIX PRO resulted in an increase in the number of side shoots by 37.5% compared to single application, while in the case of a mixture of growth regulators with a biostimulant this effect was less pronounced and amounted to 17.0%.The biostimulation treatment with Maxifruit does not guarantee improved tree growth and quality, but its use in combination with the growth regulator AGRIMIX PRO improves the effectiveness of treatments that stimulate apple branching.