The substances contained in seed shells, above-ground bodies and underground bodies - especially Juglon, which is strictly considered as natural medicinal substances, are now an alternative to synthetic preparations. In the conference will be presented and discussed influence of ethanolic walnut extract (Juglans regia L.) commonly referred as „Orzechówka” against yeast Candida albicans. The conducted experiment is the next stage of the research conducted in the field of antimicrobial properties of alcoholic walnut extract. The ethanolic extract of Juglans regia L. were tested against yeast Candida albicans ATCC 90028 obtained from the American Type Culture Collection (ATCC, distributors: LGC Standards, Łomianki, Poland). The MIC was the lowest concentration of extract which prevents visible growth of C. albicans