Behavioral responses during examinations can reflect stress and ease of handling. This study aimed to determine alpacas’ behavioral responses to body score and health assessment during physical examination, and to investigate correlations between alpacas’ behavior during physical examination (mean behavior score was computed for each animal based on the assessment of its behavioral responses: ease of introduction to the box, response to fixation, body language, vocalization, kicking, and spitting, on a 3-point scale), body condition score (BCS, on a 5-point scale) and other health indicators (on a 5-point scale), and caretaker questionnaire survey (based on evaluation of the individual’s behavioral characteristics and its ease of handling, using a 5-point Likert scale).