The aim of the study was to evaluate the possibility of using the domestic pig (Sus scrofa domesticus) as an in silico model for analyzing diseases caused by selected mutations in mitochondrial tRNA and nuclear tRNA-Met encoding genes in humans. For this purpose, the reference sequences of analogous genes in both species and the secondary structures of the molecules were compared. Nucleotide positions whose mutations are associated with the occurrence of diseases in humans were compared with analogous positions in the domestic pig. The results revealed a high degree of conservation, with nuclear tRNA genes transporting methionine achieving an average of 92% homology, while initiator tRNA values ranged from 97% to 100%. In the case of mitochondrial tRNA genes, the degree of homology varied from 64% to 93% (with an average of 80%), with the highest similarity observed for MT-TM and MT-TL2 and the lowest for MT-TV. Significantly, in nine out of the ten nucleotide positions analyzed, whose mutations in humans are associated with various disorders, the sequences in pigs were identical to their corresponding human sequences. The results obtained indicate that the high degree of tRNA sequence conservation, including the positions that determine the occurrence of diseases, may provide a basis for further research on genetic disorders associated with tRNA mutations using the domestic pig as a model organism