As an adaptive mechanism, stress response to shearing can evoke a series of changes in animals’ biological functioning, including alterations in behavioral and hormonal reactions as well as protein metabolism. The level of knowledge about alpaca stress indicators, other than behavior, cortisol, or heart rate, is still limited. The use of alternative stress indicators can provide a broader insight into an alpaca’s adaptation and welfare level. The aim of the study was to determine the effectiveness of blood protein fractions and total protein as indicators of alpacas’ stress response to shearing procedures. In Poland, twenty adult Huacaya alpacas were examined. There were four sampling days: three days before shearing, the shearing day, and the fifth and tenth days after shearing. Total protein, albumin, and globulin levels were determined. The total protein and protein fractions can be used to determine the stress response in alpacas, particularly α1- and ß1-globulins, whose levels were significantly lower on the fifth day after shearing compared to the level before shearing. Shearing induced adaptive responses in alpacas. The physiological reaction of sheared alpacas can be connected to their fleece removal and the need for thermoregulatory adaptation.