Fusariumhead blight (scab) and the contamination of cereals with mycotoxins are par-ticularly important problems in global agriculture,were the stimulus to undertake a study aimed at the determination of the share of fungi from the genus Fusariumin infecting heads of spring barley and estimation of the susceptibility of barley cultivars to F. avenaceum. The studies with 9 cultivars of spring barley were performer during three years (2020–2022). The assessment of the harmfulness of F. avenaceumfor selected cultivars of spring barley was carried out on the basis of a field exper-iment with artificial infestation of heads with a conidial suspension of the tested fungus species. Studies on fusariosis of barley heads showedthat the average percentage of heads with symptoms of infection with Fusariumspp. ranged from 0.5 to 2.0%. The dominant species on the grain of the analyzed cultivars was F. poae. F. culmorumand F. avenaceumalso played a significant role in causing fusarium head blight. Based on presented studies,it was found that inoculation of heads at the flowering stage resulted in an average reduction of 17.7% in the number of kernels per head. Onthe other hand, the average reduction of kernels yield and 1000 kernel weight for all tested cul-tivars compared to the control was 35.7% and 23.3%, respectively.The highest susceptibility to scab caused by F. avenaceumwas found for cultivar Oberek, while the Ramzes cultivar was the least susceptible