Cyclone is often used in the Industry due to its low maintenance costs, simple design, and ease of operation. This work presents both experimental and simulation evaluation on the effect of inlet velocity and mass flow rate on the performance of a wheat conveying cyclone. According to the great importance of the pressure drop and separation efficiency on the separation phenomenon in the cyclone, a comprehensive study has been conducted in this regard. A computational fluid dynamics (CFD) simulation was realized using a Reynolds stress turbulence model, and particle-air interactions were modeled using a discrete phase model. The result showed a good agreement between the measured value and CFD simulation on the pressure drop and tangential velocity with a maximum deviation of 6.8%. It was found that the separation efficiency increased with inlet velocity up to 16 m s−1 but decreased slightly at a velocity of 20 m s−1. The pressure drop increased proportionally with inlet velocity. However, optimum performance with the highest separation efficiency (99%) and acceptable pressure drop (416 Pa) was achieved at the inlet velocity of 16 m s−1 and mass flow rate of 0.01 kg s−1.