Numerous sectors of the food processing and oleochemical industries require oils with specific physicochemical properties. Fruit processing generates substantial waste potentially containing valuable raw materials for oil extraction. The significant volumes of apples and cherries processed in Poland prompted an assessment of their seeds’ suitability as oil sources. Seed dry matter, protein, and oil content were determined. The extracted oils were analyzed for acid value (AV), peroxide value (PV), oxidative stability, fatty acid composition, and sterol and tocopherol content. The predominant higher fatty acids identified in the sour cherry and sweet cherry kernel oils were linoleic acid (C18:2, n-6), with mean concentrations of 45.82% and 29.23%, respectively, and oleic acid (C18:1, n-9), accounting for 41.54% and 46.59%, respectively. Additional fatty acids detected included palmitic acid C16:0 (6.23% and 5.91%), palmitoleic acid C16:1, n-7 (0.29%), stearic acid C18:0 (1.36% and 3.11%), arachidic acid C20:0 (1.13%), α-eleostearic acid C18:3 (5.07% and 9.48%), and α-linolenic acid C18:3, n-3 (4.09%). Given the substantial proportion of the oil fraction containing numerous potentially biologically active compounds, including nutritionally valuable fatty acids, tocopherols, and phytosterols, apple, sour cherry, and sweet cherry seeds demonstrate considerable potential as raw materials for applications in the food, pharmaceutical, and cosmetics industries.