The review presents the current knowledge on the development and implementation of nanotechnology in crop production, giving particular attention to potential opportunities and challenges of the use of nano-sensors, nano-pesticides, and nano-fertilizers. Due to the size-dependent properties, e.g. high reactivity, targeted and controlled delivery of active ingredients, engineered nanomaterials (ENMs) are expected to be more efficient agrochemicals than conventional agents. Growing production and usage of ENMs result in the spread of ENMs in the environment. Because plants constitute an important component of the agri-ecosystem, they are subjected to the ENMs activity. A number of studies have confirmed the uptake and translocation of ENMs by plants as well as their positive/negative effects on plants. Here, these endpoints are briefly summarized to show the diversity of plant responses to ENMs. The review includes a detailed molecular analysis of ENMs-plant interactions. The transcriptomics, proteomics and metabolomics tools have been very recently employed to explore ENMs-induced effects in planta. The omics approach allows a comprehensive understanding of the specific machinery of ENMs occurring at the molecular level. The summary of data will be valuable in defining future studies on the ENMs-plant system, which is crucial for developing a suitable strategy for the ENMs usage