The aim of the study was to determine the titre of antibodies against canine parvovirus in groups of adult dogs from eastern Poland with a varied history of vaccination against parvovirus. 200 dogs divided into three groups were included in the study. The first group (n = 59) consisted of subjects regularly vaccinated against the disease according to WSAVA guidelines. The second group (n = 77) consisted of animals that completed the full course of CPV immunisation as puppies but had not received a booster dose in the past three years. The third group (n = 64) consisted of animals that had not received even a single vaccination against parvovirus. Blood was collected from all dogs to determine the titres of antibodies against canine parvovirus using a Bionote V200 analyser (VetExpert). In group I, antibody titres equal to or higher than HI = 80, thought of as ensuring resistance to infection were observed in 86% of dogs, while 14% of the tested animals had HI < 80. In groups II and III, high anti-CPV antibody titres of HI > 80 were found in 73% and 72% dogs, respectively. The large number of positive seroreactants with high titres of antibodies indicates the high immunogenicity of vaccine and field strains of canine parvovirus and common occurrence of canine parvovirus in the environment.